| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Holdemania filiformis | ATTTCCAAAGCGCTGGGC | TGCTTGATCGCACAGCCA | 59.03 | 59.97 | 224 | 97 |
100.00%
|
50.00%
|
| Primer 42 | Holdemania filiformis | GATTTCCAAAGCGCTGGGC | TGCTTGATCGCACAGCCA | 60.15 | 59.97 | 225 | 97 |
100.00%
|
50.00%
|
| Primer 43 | Holdemania filiformis | GTTTCAGGCTTGCTGGGGA | ATGCGATCCGTTTGGGCT | 60.23 | 59.73 | 232 | 97 |
100.00%
|
50.00%
|
| Primer 44 | Holdemania filiformis | TTTCAGGCTTGCTGGGGA | ATGCGATCCGTTTGGGCT | 58.74 | 59.73 | 231 | 97 |
100.00%
|
50.00%
|
| Primer 45 | Holdemania filiformis | TTCAGGCTTGCTGGGGATC | ATGCGATCCGTTTGGGCT | 59.70 | 59.73 | 230 | 97 |
100.00%
|
50.00%
|
| Primer 46 | Holdemania filiformis | TTCAGGCTTGCTGGGGAT | ATGCGATCCGTTTGGGCT | 58.49 | 59.73 | 230 | 97 |
100.00%
|
50.00%
|
| Primer 47 | Holdemania filiformis | AGGAGGTGGAAAGGACGGT | TGCGAGCCGATGCATTCA | 60.15 | 60.13 | 180 | 97 |
100.00%
|
50.00%
|
| Primer 48 | Holdemania filiformis | TGATGAACCGCTGCTGCA | GCCGTTGTCATAATGCGCT | 59.97 | 59.28 | 295 | 97 |
100.00%
|
50.00%
|
| Primer 49 | Holdemania filiformis | AAGCTGGCTGCCGTTTCT | CGAGCTGTGCTGATCGGAA | 59.89 | 60.15 | 271 | 96 |
100.00%
|
50.00%
|
| Primer 50 | Holdemania filiformis | AAGCTGGCTGCCGTTTCT | ATGCAGCGAGCTGTGCT | 59.89 | 59.68 | 277 | 96 |
100.00%
|
50.00%
|